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KMID : 1161520000040020151
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Animal Cells and Systems
2000 Volume.4 No. 2 p.151 ~ p.155
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Effects of nitric oxide modulating drugs on acrosome reaction in mouse spermatozoa
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Gye Myung-Chan
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Abstract
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Nitric oxide (NO) is a reactive free radical which plays important roles in animal physiology. To investigate involvement of NO in acrosome reaction (AR), effects of drugs which modulate the intracellular NO level were examined in mouse spermatozoa. N (G)?nitro?L?arginine (L?NA), a potent inhibitor of NO synthesis, decreased AR in a reversible manner. On the other hand, sodium nitroprusside (SNP), an NO generating agent, increased spontaneous AR. Preincubation of sperm in the presence of L?NA potentiated AR after sperm transfer into plain? or SNP?media. Methylene blue, a NO scavenging agent, decreased spontaneous AR. Taken together, it is concluded that NO positively controls AR.
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KEYWORD
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Nitric oxide, Acrosome reaction, Spermatozoa, Mouse
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